Schematic of immunoliposome preparation. a, Flowchart illustrating the manufacturing processes of cNB. Alpacas had been immunized with the HER2 extracellular area, and subsequent isolation of peripheral blood mononuclear cells (PBMCs) was carried out. Complete RNA was extracted from the PBMCs adopted by cDNA synthesis for amplifying variable heavy area of heavy chain (VHH) gene areas.